Interpreting MS/MS Results

Brian leads you over, under, around and through the concepts in this overview of what you can learn about peptides from mass spectrometry -- and how its done. You will learn

• Why peptides can be identified from mass spectra.
• What motivated the development of Sequest.
• How Mascot and X! Tandem differ from Sequest.
• Why combining results from searching the same data with different programs is a good idea.
• One way of doing this combining.

Brian's power point presentation is on the right.
The sidebar on the right provides links to the software discussed in the power point presentation.

PepSeq - Online Peptide Sequencing Simulation Software

Once you've got the general idea from Brian's power point slides, you might want to investigate the online peptide sequencing simulation PepSeq by M.J. Robinson at Monash University in Gippsland, Victoria, Australia.